to identify neurons in drosophila that when activated would change walking direction.​ ​ approach:​ the researchers used a uastrpa1 transgene that targeted expression of trpa1 to certain sets of neurons in drosophila. trpa1 is a cation channel which is thermosensitive and allowed them to control expression by changing the temperature. they tested this with vt-gal4/uas-trpa1 and uas-trpa1 for a control. they put these drosophila into a ring assay at 30 degrees celsius. they also put drosophila at 22 degrees celsius to act as a control. then, they tracked the position and how often each drosophila walked forward and backward.​ ​ results:​ for the ring assay, vt50660-gal4/uas-trpa1 drosophila mostly walked at 30 degrees celsius. they would rarely walk backwards when at 22 degrees celsius. uas-trpa1 drosophila walked mostly forward at 22 degrees celsius and at 30 degrees celsius.​ ​ conclusions:​ the neurons that are targeted in the line vt50660-gal4/uas-trpa1 trigger backwards walking when activated. the results of vt50660-gal4/uas-trpa1 walking backwards at 30 degrees celsius supports this conclusion because backwards walking is induced from the neurons being activated by the thermosensitive channel.