You are given a plasmid. In order to map this plasmid, you do a series of restriction enzyme digestions and obtain the following results on agarose gel electrophoresis (M1 and M2 are size markers):
a. What is the approximate size of this plasmid?
b. Create a circular plasmid map showing the relative positions of the BamH1, Sma1, Kpn1 and Bgl2 sites. Show the distances in bp between each of the adjacent sites.