Krüppel binds to the consensus DNA sequence AACGGGTTAA. Which of the following amino acid substitutions would likely change the binding specificity of the protein so it binds to a DNA sequence different from the consensus sequence?
1) Substitution of Alanine with Valine
2) Substitution of Glycine with Proline
3) Substitution of Threonine with Serine
4) Substitution of Lysine with Arginine