What would occur if after incubating the ELISA plate, the wells were not washed with PBS and the unbound primary SLE antibody is not removed?
A) A false positive result because the secondary anti-human antibody will not bind to the unbound primary SLE antibody.
B) A false positive result because the secondary anti-human antibody will bind to the primary SLE antibody.
C) A false negative result because the secondary anti-human antibody will bind to the primary SLE antibody.
D) A false negative result because the secondary anti-human antibody will bind to the primary SLE antibody, regardless if the primary antibody is bound to antigen or not.