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Assuming that transcription and translation both proceed from left to right, which is the correct orientation of the DNA template, the RNA transcript, and the protein product? DNA template 3'– ……… –5'RNA transcript 5'– ……… –3'Protein product HOOC– ……. ..–NH2DNA template 3'– ……… –5'RNA transcript 5'– ……… –3'Protein product H2N– ……. –COOHDNA template 5'– ……… –3'RNA transcript 5'– ……… –3'Protein product H2N– ……. –COOHDNA template 5'– ……… –3'RNA transcript 3'– ……… –5'Protein product HOOC– ……. ..–NH2

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The correct answer is:

DNA template 3'– ……… –5'

RNA transcript 5'– ……… –3'

Protein product H2N– ……. –COOH

In nucleic acids, DNA and RNA 5’ end refers to phosphate group attached to the 5′ carbon of the ribose ring, while 3’ end refers to the ribose -OH substituent. Nucleic acids can only be synthesized in the 5′-to-3′ direction, which means that the DNA template is 3’—5’. Translation of the protein by the ribosome will also proceed in a 5′ to 3′ direction, which means that the mRNA template is 3’—5’.

The protein during the synthesis is extend from its N terminus toward its C terminus.

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