Answer:
be cut by the same restriction enzyme, resulting in the formation of complementary sticky ends
In order to insert a human gene into a plasmid, both must be cut by the same restriction enzyme.
A restriction enzyme is a protein that cleaves specific nucleotide sequences in the DNA molecule.
During recombinant DNA techniques, both target DNA (i.e., the genetic sequence to be modified) and the gene sequence to be inserted are cleaved by restriction enzymes that cut at the same nucleotide sequence.
Subsequently, a specific enzyme called ligase catalyzes the joining (i.e., the ligation) of both nucleotide sequences by forming a new phosphodiester bond.
In conclusion, in order to insert a human gene into a plasmid, both must be cut by the same restriction enzyme.
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