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Which of the following are typically used to construct a cDNA library? Choose all that apply.Reverse transcriptasePrimary mRNA transcriptsDNA ligaseWhole cell DNADNA polymerase

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BioWizard

Answer:

Reverse transcriptase

DNA polymerase

DNA ligase

Explanation:

cDNA library consists of cloned cDNA (complementary DNA) inserted into a host. cDNA is produced from the mature mRNA (transcriptome form the nucleus) via reverse transcriptase (synthesize single stranded DNA from RNA). Single stranded cDNA is converted into a double stranded DNA thanks to DNA polymerase.  Restriction endonucleases and DNA ligase are enzymes necessary for the cloning process ( for cloning the sequences into host-bacterial plasmids).

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