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Arrange the following list of eukaryotic gene elements in the order they would appear in the genome and in the direction traveled by RNA polymerase along the gene. Assume the gene's single intron interrupts the open reading frame. Note that some of these names are abbreviated and thus do not distinguish between elements in DNA versus RNA. For example, "splice-donor site" is an abbreviation for "DNA sequences transcribed into the splice-donor site" because splicing takes place on the gene's RNA transcript, not on the gene itself. Geneticists often use this kind of shorthand for simplicity, even though it is imprecise. (a) splice-donor site; (b) 3' UT R; (c) promoter; (d) stop codon; (e) nucleotide to which methylated cap is added; (f) initiation codon; (g) transcription terminator; (h) splice-acceptor site; (i) 5' UT R; (j) poly-A addition site; (k) splice branch site.

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Answer:

The alignment of the elements in the following sequence will take place in the eukaryotic genome:  

a. Promoter

b. Nucleotide to which methylated cap is added

c. 5 prime UTR

d. Initiation codon

e. Splice donor

f. Splice branch site

g. Splice acceptor

h. Stop codon

i. 3 prime UTR

j. Transcription terminator

k. Poly A addition site

After the process of splicing, the ultimate transcript will comprise the elements b, c, d, h, i. In eukaryotes, the RNA polymerase begins the process of transcription after it crosses the promoter region, and ceases at the transcription terminator. At the time of RNA processing, a 5 prime cap is supplemented to the transcript, splicing occurs, and a poly-A tail is supplemented. The 5 prime UTR and 3 prime UTR regions are found in the final transcript, that is, the mature RNA, however, are not translated.  

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