PCR utilizes specific DNA primers and a thermo stable polymerase enzyme to amplify (make many copies of) a particular DNA sequence (typically 100-600 bases long). Double stranded DNA (dsDNA) must first be separated and primers anneal (complimentary base pair to) their targeted sequences. Polymerase can then extend the DNA strands. What must also be added to the mixture besides primers and polymerase enzymes in order to have amplification of DNA?A.ATP.
B.RNA polymerase.
C.dNTPs.
D.electron carriers.
E.DNA repair enzymes.

dNTP stands for deoxynucleoside triphosphate. They are composed of a sugar, a nitrogenous base and three phosphate groups and they are used in PCR reactions as the monomers of DNA. There are four of them (dATP, dTTP, dGTP and dCTP), each containing a different nitrogenous base (adenine, thymine, guanine or cytosine respectively).

When the DNA polymerase extends the primers, it adds a dNTP complementary to the template strand. If dNTP were not added to the mix, no nucleotides would be available to synthesize new strands and no amplification would happen.