A microscope technique that will not allow one to see specific parts of living specimen is

A) using stain under a light microscope

B) using a Scanning Electron Microscope

C) passing light from a microscope through a pinhole

D) using a fluorescent substance with a specimen under a light microscope

I know that sometimes with using a stain the specimen can be killed, but im not sure if thats always the case. Thanks in advance for your help!!