The first step in annotating a gene is identifying the open reading frame. In bacteria, all genes that code for a protein have a start and stop codon with an open reading frame between them. A bioinformatics program can translate all the possible reading frames (series of 3 codons) in an attempt to find the longest open reading frame.

How many possible open reading frames can a bioinformatics program translate from one DNA sequence? Enter your answer numerically (for example: 2).

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Answer: 6

Explanation:

A DNA strand has 3 distinct frame, thus for both strands, it will require 6 strands.