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The table represents the results of an experiment where the effects of pH buffers on an enzyme found in saliva (amylase) were studied. A spectrophotometer set at 500 nm was used to measure absorbance at the various pH levels every 20 seconds for 2 minutes. The higher absorbance values would indicate greater enzyme activity. All experiments were conducted at the same temperature. Which statement correctly identifies the result that the optimum pH for amylase function is 7?

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Answer:

The amylase had the higher absorbance with a pH of 7.

Explanation:

Enzyme activity values ​​are given by the spectrophotometric method. The constant temperature is maintained in order to evaluate the pH independent effect. The absorbance is then measured at various pHs and the one that corresponds to the greatest absorbance is the pH at which the enzyme has its peak activity. So pH 7 is the optimal pH condition.

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