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When recovering the aqueous and organic layers from the separatory funnel ______. (Select all that apply.) A. the lower layer should be allowed to drain through the tap B. the stopper must be removed in order to allow the funnel to drain C. the stopper must be kept in place during draining D. the aqueous layer should always be poured from the top, regardless of which layer it is E. magnesium sulfate should be added to the funnel before pouring, to dry the organic layer

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Answer: Option A and C applies

- A. the lower layer should be allowed to drain through the tap

- C. the stopper must be kept in place during draining

Explanation:

Since aqueous and organic substances do not mix, they form a layer between them. Hence, require a separatory funnel with a stopper to recover each fraction effectively.

First, the lower fraction (aqueous) is recovered when the stopper is opened to allow draining through the tap into a well labelled beaker

Second, the stopper must be kept in place to shut the tap once the lower fraction has been collected. So, the organic fraction remains in the seperating funnel

Thus, Option A and C applies

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