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In the active site of the enzyme lotzase, a mutation of lysine 132 toaspartic acid causes a loss of lotzase function. Lotzase is most catalytically active at pH 7. Which is the following is the best reason why this might occur?
a. a negative charge is needed for substrate binding.
b. a positive charge is needed for substrate binding.
c. a general acid is needed for catalysis to occur.
d. more than one of the following can be a good reason.

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Answer:

B. positive charge is needed for substrate binding

C. a general acid is needed for catalysis to occur

Explanation:

A mutation of lysine 132 toaspartic acid causes a loss of lotzase function as a result of the enzyme lotzase being most catalytically active at pH 7( neutral).

This condition is however achieved under conditions such as a positive charge being involved in the substrate binding and a general acid being involved in the catalytic process.