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You have just trypsinized your monolayer cells growing in a T25 flask. Once detached you resuspended all of your cells in media. If you were to subculture some of your cells into a new T25 cells by taking 1.5 mL of cell suspension from a total of 3 mL and transferring it into a new T25 flask containing 8 mL of fresh media, your final dilution would be:

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Answer:

Final dilution is   [tex]\frac{1}{6.33}[/tex]

Explanation:

Given

Volume of suspension [tex]= 3[/tex] mL

Volume of subculture [tex]= 1.5[/tex] mL

Volume of fresh media [tex]= 8[/tex] mL

Final dilution

[tex]\frac{1.5}{8+1.5}[/tex]

Final dilution

 [tex]= \frac{1.5}{9.5}\\\\= \frac{15}{95} \\= \frac{1}{6.33}[/tex]

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