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The analytic result of RDR 1/RDR 2/RDR 6-independent small RNA is given below.
what are interfering RNA loci?
RNAi is a powerful, effective, and well-characterized method for gene silencing for loss-of-function studies. This technology is proven in the elucidation of biological pathways, disease gene analysis, and target gene discovery.
Plant short RNAs (sRNAs) control important physiological processes by repressing gene expression both transcriptionally and post-transcriptionally. Small RNAs can be broadly divided into two groups: those whose biosynthesis depends on RNA-dependent RNA polymerases (RDRs) and those whose biogenesis does not. Phased and repeat-associated short interfering RNAs are examples of known RDR1/2/6-dependent sRNAs, whereas microRNAs (miRNA) and other sRNAs produced from hairpins are examples of known RDR1/2/6-independent sRNAs. In this study, we created and examined sRNA-seq libraries from triple mutant rdr1/rdr2/rdr6 plants. We disproved the classification of 58 previously classified miRNA loci by discovering that they depended on RDR1, -2, or -6 function. 38 RDR1/2/6-independent sRNA loci that are not miRNAs or otherwise hairpin-derived and do not fit into any of the other established models for sRNA biogenesis were also discovered by us. These 38 loci that produce sRNA.
At Thermo Fisher Scientific, we have developed a broad suite of tools for RNAi applications, including short interfering RNA (siRNA) for targeted gene knockdown and microRNA (miRNA) reagents to mimic or inhibit endogenous gene regulators. These market-leading reagents are suitable for in vivo or in vitro research, including high-throughput screening with RNAi libraries. Learn more about how we can help with end-to-end solutions for the entire RNAi workflow.
To learn more about RNA loci with the help of the given link:
https://brainly.com/question/28212315
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