Respuesta :
We provide a proteomic analysis of microdissected colorectal cancer tissue that was formalin-fixed and paraffin-embedded, measuring > 7500 proteins between patient matched normal mucosa, primary carcinoma, and nodal metastases.
- Compared to transcript-based research, the expression levels of 1808 proteins dramatically varied between normal and malignant tissues by a far bigger percentage.
- The cell-surface and nuclear proteomes are greatly altered in tumor cells.
- Comparing the proteome alterations in rapidly expanding and differentiated CaCo-2 cells, functionally comparable modifications were found.
- Between primary cancer and metastases, however, there was comparatively little proteomic remodeling, indicating that just minor proteome modifications are required for the tumor to spread to a different tissue setting.
- We also present a novel approach to estimate protein copy numbers per cell in the absence of protein standards.
- The estimated copy numbers in enterocytes and cancer cells are in good accord with the data from the literature, CaCo-2, and HeLa cells.
- Additionally, the quantitative alterations of functional protein classes can be mapped using the proteomic data set we have, providing new insights into the biology of colon cancer.
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