To disrupt the covalent disulfide bonds and mercaptoethanol to overcome the noncovalent interchain bonds.
A chemical species known as a reducing agent in chemistry is one that "donates" an electron to an electron recipient (also known as a reductant, reducer, or electron donor) (called the oxidizing agent, oxidant, oxidizer, or electron acceptor)
A reducing substance undergoes oxidation because of the redox reaction's electron loss. The metals found in the earth, formic acid, and sulfite compounds are a few examples of reducing agents. A reducing agent is a material that, in a redox process, loses electrons to other substances and undergoes oxidation to a higher valency state.
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