Substrate-level phosphorylation is a term given to product formation resulting in ADP or GDP phosphorylation.
Substrate-level phosphorylation is the reaction in which a phosphate group gets transferred directly from any substrate to ADP or GDP. It occurs during the glycolysis process in the cytoplasm, and also in the Krebs cycle.
Phosphorylation is the addition of a phosphate group to any compound. It usually gets transferred from one compound to another. This process is mediated with the help of a class of enzyme called 'kinases'. The opposite process of removing phosphate group is called dephosphorylation and is mediated by the enzyme phosphatases.
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