45gram of ampicillin would be needed.
Final concentration of ampicillin solution = 100mg/ml
It means that 1 ml of solution will have 100 mg ampicillin
So, 450 ml of solution will have = 450 * 100 = 45000 mg ampicillin
45000 mg = 45 gram
Hence, to make 450 ml of solution 45 gram of ampicillin would be needed.
Ampicillin: at a final concentration of 100ug/ml, add 1 ml of ampicillin (at a concentration of 100 mg/ml) per liter of agar.
Add 10 mL of d H2O to 1 g of Amp powder.
When powder is completely dissolved, vortex.
Use a sterile 0.22 mm filter to sterilize.
Store at -20 °C and aliquot 1 mL of the amp solution into a 1.5 mL tube.
How is ampicillin 50 mg/mL produced?
Ampicillin sodium salt 500 mg can be dissolved in water to a final volume of 10 ml to create a 50 mg/ml stock solution.
With 1 mL, 1.8 mL, 3.5 mL, and 6.8 mL of each of the following: 250 mg, 500 mg, 1 g, and 2 g vials
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