Mendel’s monohybrid cross with round and wrinkled seeds and other single traits tested, directly contradict the blending hypothesis. The result of the cross between round and wrinkled pure breed cell lines was all round seeds in the F1 offspring, not partially round and wrinkled as blending theory would predict.
Mendel discovered that the round-seeded and wrinkled-seeded traits were two facets of the same character, suggesting to modern plant scientists that a single gene determines whether seeds are round or wrinkled.
In a monohybrid cross, neither allele is blended; both alleles are expressed in the F2 generation. The fact that each gamete only contains one allele serves as the foundation for the law of segregation.
Mendel discovered through monohybrid crossings that while distinct alleles having the potential to affect a single characteristic, they remained indivisible and could be inherited individually. Additionally, the allele could exist but be undetectable in one generation before resurfacing in the following generation.
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