To arrest all the transcriptional events at the same position on the DNA template, the sequence of the gene being transcribed must be known, and in the reaction mixture three rNTPs must be added except for the one where the transcription process need to be arrested.
rNTPs are the Ribonucleoside tri-phosphates. It is comprised of a ribose sygar joined to three phosphate groups. There are 4 types of rNTPs: rATP, rCTP, rUTP, rGTP. They are required for making the RNA strand from DNA.
Transcription is the process of synthesizing the RNA strand from the template DNA strand. The process occurs in the nucleus of the eukaryotic cells and in the nucleiod of prokaryotes.
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