The prediction at those two positions in a newly sequenced gene is Position 1 (T) and position 2 (G).
They always bring about changes in the genotype of an organism. Regardless of the cause of the change, the DNA sequence will inevitably change when mutated. Sequence logos help predict where certain gene features are located. Gene features such as ribosome binding sites and initiation codons occur in highly conserved portions of the DNA sequence.
In other words, base positions with high letter stacks probably represent features common to a large number of genes. Sequence logos are graphic representations of nucleotide or amino acid sequence conservation. Sequence logos are created from a collection of aligned sequences to show consensus sequences and sequence diversity.
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