In yeast, LYS5, ADE1, and URA2 are genes required to synthesize lysine, adenine and uracil, respectively. A MATa lys5 ADE1 URA2 haploid was mated to a MATA LYS5 ade1 ura2 haploid to make a triply heterozygous diploid. This diploid was put through meiosis, 100 tetrads were dissected and the spore colonies transferred to medium lacking either lysine, adenine or uracil to determine which spores were prototrophic or auxotrophic for those nutrients. The following results were obtained:
PD NPD I
LYS5-ADE1 20 22 58
ADE1-URA2 18 20 62
LYS5-URA2 50 8 42
Based on this information, select the statements below that are TRUE.
A) The LYS5 and URA2 genes are linked.
B) The genotypes of the four spores in the ADE1-URA2 tetratypes is
ade1 ura2
ade1 URA2
ADE1 ura2
ADE1 URA2
C) The map distance between ADE1 and URA2 is 51 CM.
D) ADE1 and URA2 are likely located on the same chromosome.
E) The map distance between LYS5 and URA2 is 29 CM.
F) LYS5 and ADE1 independently assort during meiosis.
G) The genotypes of the four spores in the LYS5-URA2 NPDS are:
lys5 URA2
lys5 URA2
LYS5 ura2
LYS5 ura2
H) LYS5 and URA2 are likely located on the same chromosome.