Spectrophotometry is a laboratory technique that can be performed first to check if there is presence of arsenic mixed in with Gatorade. In spectrophotometry, arsenic concentration will be measure in a specific solution by calculating the amount of light absorbed by inorganic arsenic.
Various analytical reagents are used such as molybdate reagent and variamine blue as a chromogenic reagent where a violet colored indicates presence of arsenic.
Furthermore, new analytical reagents has been development and proposed like vanillin-2- amino nicotinic acid (VANA) and 2, 4-Dihydroxy benzophenone-2-amino thiophenol (BPBT). These are used for direct non-extractive spectrophotometric determination of arsenic. The reagent reacts with arsenic in acidic medium to form light greenish yellow colored.