Respuesta :
Nitrogen in the imidazole ring possesses hydrogen but no charge; much lower than the pH values typically found in a cell.
Is imidazole acidic or basic?
Imidazole is amphoteric; it can act as both acid and base. The compound is classified as aromatic due to the presence of π-electrons, consisting of a pair of electrons from the protonated nitrogen atom and one from each of the remaining four atoms of the ring.
What is the function of imidazole?
Imidazole is used as a competitive agent for the release of histidine-containing proteins. In addition, imidazole can be added in low concentrations in the sample and binding buffer to reduce the binding of contaminant proteins, thus increasing the final purity.
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