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In situ hybridization is a powerful tool used in gene expression studies because it provides the investigator with information pertaining toA)the cellular and tissue-specific localization of the mRNA encoded by a particular gene.B)the activity of the protein translated from a particular mRNA.C)the size of the mRNA transcript.D)all of the above

Respuesta :

In situ hybridization

Explanation:

A)the cellular and tissue specific localization of the mRNA encoded by a particular gene

  • In situ hybridization is a technique used to locate and detect nucleic acid sequences(DNA or RNA) with respect to their protein product within nuclei
  • The principle behind in situ hybridization is that specific annealing(heat treatment process) of labelled probe to complementary sequences of target DNA or mRNA in a fixed specimen is done
  • Probe is mainly used to find the complementary sequence of the nucleic acids(DNA or RNA) or helps in localization of particular clone
  • The detection and visualization of the hybrids can be done by using cytological methods